In vitro response of Blastocystis hominis against traditional Chinese medicine
Identifieur interne : 002995 ( Main/Exploration ); précédent : 002994; suivant : 002996In vitro response of Blastocystis hominis against traditional Chinese medicine
Auteurs : L. Q. Yang [République populaire de Chine] ; Mulkit Singh [Singapour] ; E. H. Yap [Singapour] ; G. C. Ng [Singapour] ; H. X. Xu [Singapour] ; K. Y. Sim [Singapour]Source :
- Journal of Ethnopharmacology [ 0378-8741 ] ; 1996.
English descriptors
- Teeft :
- Active concentration, Blastocystis hominis, Cell count, Cell counts, Cell rate, Control group, Coptis chinensis, Crude extracts, Dead cells, Different drugs, Drug concentration, Herbal extracts, High inhibition, Hominis, Intact cells, Intestinal parasite, Light microscope, Metronidazole, Moderate inhibition, Percentage rate, Present study, Shanghai university, Traditional chinese medicine, Traditional medicine, Vacuolar forms, Zierdt.
Abstract
Abstract: This is the first in vitro study on the activity of 20 kinds of crude extracts of traditional Chinese medicine (TCM) on the intestinal parasite, Blastocystis hominis using the criteria of living cell count (LCC) and living cell rate (LCR). LCC and LCR were applied as observation indicators, the former as a fixed-quantity and the latter as a fixed-quality method. LCR calculated percentage rate of living cells using eosin-brilliant cresyl blue staining which could differentiate between living cells and dying or dead cells. There were five extracts with no inhibitory activity, thirteen with moderate inhibition and two with high inhibition. The crude extracts of Coptis chinensis (CC) and Brucea javanica (BJ) were found to be most active against B. hominis. The active concentration of CC was 100 μg/ml. The active concentration of BJ was 500 μg/ml. The active concentration of metronidazole (MD) was 10 μg/ml and this was taken as an active standard drug for B. hominis.
Url:
DOI: 10.1016/S0378-8741(96)01471-7
Affiliations:
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Q." last="Yang">L. Q. Yang</name><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Department of Parasitology, Shanghai University of Traditional Chinese Medicine, 530 Ling Ling Road, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation></author><author><name sortKey="Singh, Mulkit" sort="Singh, Mulkit" uniqKey="Singh M" first="Mulkit" last="Singh">Mulkit Singh</name><affiliation></affiliation><affiliation wicri:level="4"><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Microbiology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Singapour</country></affiliation></author><author><name sortKey="Yap, E H" sort="Yap, E H" uniqKey="Yap E" first="E. H." last="Yap">E. H. Yap</name><affiliation wicri:level="4"><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Microbiology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author><author><name sortKey="Ng, G C" sort="Ng, G C" uniqKey="Ng G" first="G. C." last="Ng">G. C. Ng</name><affiliation wicri:level="4"><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Microbiology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author><author><name sortKey="Xu, H X" sort="Xu, H X" uniqKey="Xu H" first="H. X." last="Xu">H. X. Xu</name><affiliation wicri:level="4"><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Chemistry, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author><author><name sortKey="Sim, K Y" sort="Sim, K Y" uniqKey="Sim K" first="K. Y." last="Sim">K. Y. Sim</name><affiliation wicri:level="4"><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Chemistry, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Ethnopharmacology</title><title level="j" type="abbrev">JEP</title><idno type="ISSN">0378-8741</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996">1996</date><biblScope unit="volume">55</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="35">35</biblScope><biblScope unit="page" to="42">42</biblScope></imprint><idno type="ISSN">0378-8741</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0378-8741</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Active concentration</term><term>Blastocystis hominis</term><term>Cell count</term><term>Cell counts</term><term>Cell rate</term><term>Control group</term><term>Coptis chinensis</term><term>Crude extracts</term><term>Dead cells</term><term>Different drugs</term><term>Drug concentration</term><term>Herbal extracts</term><term>High inhibition</term><term>Hominis</term><term>Intact cells</term><term>Intestinal parasite</term><term>Light microscope</term><term>Metronidazole</term><term>Moderate inhibition</term><term>Percentage rate</term><term>Present study</term><term>Shanghai university</term><term>Traditional chinese medicine</term><term>Traditional medicine</term><term>Vacuolar forms</term><term>Zierdt</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: This is the first in vitro study on the activity of 20 kinds of crude extracts of traditional Chinese medicine (TCM) on the intestinal parasite, Blastocystis hominis using the criteria of living cell count (LCC) and living cell rate (LCR). LCC and LCR were applied as observation indicators, the former as a fixed-quantity and the latter as a fixed-quality method. LCR calculated percentage rate of living cells using eosin-brilliant cresyl blue staining which could differentiate between living cells and dying or dead cells. There were five extracts with no inhibitory activity, thirteen with moderate inhibition and two with high inhibition. The crude extracts of Coptis chinensis (CC) and Brucea javanica (BJ) were found to be most active against B. hominis. The active concentration of CC was 100 μg/ml. The active concentration of BJ was 500 μg/ml. The active concentration of metronidazole (MD) was 10 μg/ml and this was taken as an active standard drug for B. hominis.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li><li>Singapour</li></country><orgName><li>Université nationale de Singapour</li></orgName></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Yang, L Q" sort="Yang, L Q" uniqKey="Yang L" first="L. Q." last="Yang">L. Q. Yang</name></noRegion></country><country name="Singapour"><noRegion><name sortKey="Singh, Mulkit" sort="Singh, Mulkit" uniqKey="Singh M" first="Mulkit" last="Singh">Mulkit Singh</name></noRegion><name sortKey="Ng, G C" sort="Ng, G C" uniqKey="Ng G" first="G. C." last="Ng">G. C. Ng</name><name sortKey="Sim, K Y" sort="Sim, K Y" uniqKey="Sim K" first="K. Y." last="Sim">K. Y. Sim</name><name sortKey="Singh, Mulkit" sort="Singh, Mulkit" uniqKey="Singh M" first="Mulkit" last="Singh">Mulkit Singh</name><name sortKey="Xu, H X" sort="Xu, H X" uniqKey="Xu H" first="H. X." last="Xu">H. X. Xu</name><name sortKey="Yap, E H" sort="Yap, E H" uniqKey="Yap E" first="E. H." last="Yap">E. H. Yap</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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